Thermo Fisher Scientific EEA1 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt.

Target Information

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane-bound protein specific to the early endosome and essential for fusion between early endocytic vesicles. Early endosomes fuse with endocytic vesicles to redistribute extracellular compounds.
EEA1 contains zinc-finger-like domains in both amino and carboxyl-terminal regions, similar to those found in nucleic acid binding proteins. The carboxyl-terminal zinc-finger-like domain is conserved among several non-nuclear proteins involved in intracellular trafficking.
This authentic zinc-binding domain is critical for intracellular localization of EEA1. Membrane association of EEA1 depends on phosphatidyl 3-kinase activity; inhibitors cause dissociation from early endosomes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.