Thermo Fisher Scientific EEA1 Polyclonal Antibody
EEA1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, IP 등 다양한 응용에 사용 가능. 4°C에서 보관하며, 액상 형태로 제공. 세포 내 초기 엔도솜 융합 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 100 and 150 of human early endosome antigen 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
Early endosomal antigen 1 (EEA1) is a 162 kDa membrane-bound protein specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes fuse with endocytic vesicles to redistribute extracellular compounds to alternate destinations.
EEA1 contains zinc-finger-like domains in both amino and carboxyl-terminal regions, which are conserved in several other non-nuclear proteins involved in intracellular trafficking. The carboxyl-terminal zinc-binding domain is critical for EEA1 localization, and its membrane association depends on phosphatidyl 3-kinase activity. Inhibitors of this kinase cause EEA1 to dissociate from early endosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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