Thermo Fisher Scientific EEA1 Polyclonal Antibody
EEA1 단백질을 인식하는 Rabbit Polyclonal Antibody로 인간 및 생쥐 시료에 반응. Western blot과 Immunoprecipitation에 적합. 항원 친화 크로마토그래피로 정제된 액상 형태. 4°C에서 보관하며 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:10,000
Immunoprecipitation (IP)
- Tested Dilution: 2–5 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 175 and 225 of human early endosome antigen 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
Target Information
Early endosomal antigen 1 (EEA1) is a 162 kDa membrane-bound protein specific to the early endosome and essential for fusion between early endocytic vesicles. Early endosomes fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations.
EEA1 contains zinc-finger-like domains in both amino and carboxyl-terminal regions, similar to those found in nucleic acid-binding proteins. The carboxyl-terminal zinc-finger-like domain is conserved among several non-nuclear proteins involved in intracellular trafficking.
This domain is an authentic zinc-binding domain critical for EEA1 localization. Membrane association of EEA1 depends on phosphatidyl 3-kinase activity; inhibitors cause EEA1 to dissociate from early endosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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