Thermo Fisher Scientific SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), eFluor 660, eBioscience
SSEA-4 항원을 인식하는 Mouse monoclonal antibody로, eFluor 660 형광 표지형입니다. 인간 및 다양한 종의 줄기세포 연구에 적합하며, Flow Cytometry 및 Immunocytochemistry에 사용 가능합니다. 4°C 보관, 동결 금지.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), eFluor 660, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 4 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Chicken, Human, Mouse, Non-human primate, Rabbit |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioMC-813-70 (MC-813-70) |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574296 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Biotin
- PE
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein.
Expression is found on human teratocarcinoma stem cells (EC), embryonic germ cells (EG), induced pluripotent stem (iPS) cells, and embryonic stem cells (ES), with decreased expression upon differentiation. Expression has also been observed on bone marrow mesenchymal stem cells.
This antibody cross-reacts with mouse, rabbit, canine, chicken, and Old and New World monkeys.
Applications Reported: Flow cytometric analysis and immunocytochemistry.
Applications Tested:
- Flow Cytometry: ≤0.5 µg/test (in 100 µL volume, 10⁵–10⁸ cells/test)
- Immunocytochemistry: ≤10 µg/mL
It is recommended to titrate the antibody for optimal performance.
Fluorochrome Information:
eFluor® 660 is a replacement for Alexa Fluor® 647.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen expressed along with SSEA-3, TRA-1-60, and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS).
In human cells, these surface markers are downregulated during differentiation, while SSEA-1 is absent in undifferentiated human stem cells and upregulated after retinoic acid–mediated differentiation.
Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation, while SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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