Thermo Fisher Scientific SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), eFluor 660, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Biotin
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein.
Expression is found on human teratocarcinoma stem cells (EC), embryonic germ cells (EG), induced pluripotent stem (iPS) cells, and embryonic stem cells (ES), with decreased expression upon differentiation. Expression has also been observed on bone marrow mesenchymal stem cells.

This antibody cross-reacts with mouse, rabbit, canine, chicken, and Old and New World monkeys.

Applications Reported: Flow cytometric analysis and immunocytochemistry.
Applications Tested:

• Flow Cytometry: ≤0.5 µg/test (in 100 µL volume, 10⁵–10⁸ cells/test)
• Immunocytochemistry: ≤10 µg/mL

It is recommended to titrate the antibody for optimal performance.

Fluorochrome Information:
eFluor® 660 is a replacement for Alexa Fluor® 647.

• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen expressed along with SSEA-3, TRA-1-60, and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS).
In human cells, these surface markers are downregulated during differentiation, while SSEA-1 is absent in undifferentiated human stem cells and upregulated after retinoic acid–mediated differentiation.
Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation, while SSEA-3 and SSEA-4 expression increase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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