Thermo Fisher Scientific TWIST1 Polyclonal Antibody
TWIST1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC(P), ELISA에 사용 가능. Human, Mouse, Rat 반응성. 핵 내 단백질 검출에 적합하며, 2.23 mg/mL 농도의 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–100 of Twist (NP_0004651) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.23 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914435 |
Product Specific Information
- Positive test controls: Rat heart
- Subcellular location: Nucleus
Immunogen sequence:
MMQDVSSSPV SPADDSLSNS EEEPDRQQPP SGKRGGRKRR SSRRSAGGGA GPGGAAGGGV GGGDEPGSPA QGKRGKKSAG CGGGGGAGGG GGSSSGGGSP
Target Information
TWIST1 acts as a transcriptional regulator. It inhibits myogenesis by sequestering E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction.
TWIST1 also represses proinflammatory cytokines such as TNFA and IL1B. It regulates cranial suture patterning and fusion, and gene expression varies depending on dimer composition.
Mutations in the TWIST1 gene are implicated in Saethre-Chotzen syndrome, characterized by coronal synostosis, brachycephaly, low front hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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