Thermo Fisher Scientific SRSF4 Polyclonal Antibody
Rabbit polyclonal antibody targeting human SRSF4 (SRp75) for WB and IHC applications. Detects endogenous 57 kDa protein with >95% purity. Suitable for human, mouse, and rat samples. Provided in PBS with glycerol, stored at 4°C or -20°C.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
- View 1 publication
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
- View references
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 100–150 of human SRp75 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553494 |
Product Specific Information
- Detects endogenous protein at a molecular weight of 57 kDa.
- Purity >95% by SDS-PAGE.
Target Information
Serine/arginine-rich protein 75 (SRp75) is encoded by the SRSF4 gene. SRp75 belongs to the arginine/serine-rich factor family and likely plays a role in alternative mRNA splicing.
SR factors contain one or more RNA recognition motifs (RRM) and an arginine/serine (RS)-rich domain. They are essential for constitutive splicing and regulate splicing in a concentration-dependent manner by influencing alternative splice site selection.
Most SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 shuttle between the nucleus and cytoplasm, contributing to mRNA transport.
The activity of SR proteins in regulated splicing is antagonized by hnRNP A/B family members, which induce shifts in splice site selection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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