Thermo Fisher Scientific NEFM Recombinant Rabbit Monoclonal Antibody (ARC0396)
Rabbit 모노클로날 항체로 인간 Neurofilament M 단백질을 특이적으로 인식. Western blot, ICC/IF, ELISA에 사용 가능. HEK293 세포에서 발현된 재조합 항체로 높은 특이성과 재현성 제공. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0396 |
| Immunogen | Synthetic peptide corresponding to amino acids 600–700 of human Neurofilament M (P07197) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849155 |
Product Specific Information
Immunogen sequence:
ELVADAKVEK PEKAKSPVPK SPVEEKGKSP VPKSPVEEKG KSPVPKSPVE EKGKSPVPKS PVEEKGKSPV SKSPVEEKAK SPVPKSPVEE AKSKAEVGKG E
Target Information
Neurofilaments (NFs) are intermediate filaments expressed almost exclusively in neuronal cells, especially in large axons. They consist of three polypeptide chains—NF-M, NF-H, and NF-L—that share structural similarity but differ in terminal sequences. NF-M tail-mediated interactions are essential for axonal size and cytoskeletal organization. NF-M phosphorylation and O-GlcNAcylation are reciprocally regulated, influencing filament formation and function. Antibodies against NF subunits serve as valuable neuronal markers due to their abundance, neuron-specific expression, and biochemical stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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