Thermo Fisher Scientific Palladin Monoclonal Antibody (1E6)
Palladin 단백질을 인식하는 Mouse monoclonal antibody (clone 1E6). Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. Human, Mouse, Rat 등 여러 종에 반응. Protein G 정제, 1 mg/mL 농도의 액상 형태. 세포 골격 연구 및 actin 관련 연구용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:20–1:100 | - |
| Flow Cytometry (Flow) | 5 µg/mL | 1 publication |
| Immunoprecipitation (IP) | 0.05 mL/2 mL lysate | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Chicken, Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1E6 |
| Immunogen | Endogenous Palladin immunoprecipitated from chicken embryo fibroblasts |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | TBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11152174 |
Product Specific Information
- Suggested positive control: MDA-MB231 (human breast cancer) lysates.
Target Information
Palladin is a cytoskeletal protein required for organization of the normal actin cytoskeleton. It plays key roles in establishing cell morphology, motility, adhesion, and interactions with the extracellular matrix across various cell types. Palladin may act as a scaffolding molecule influencing actin polymerization and the assembly of actin filaments into higher-order structures. It binds to proteins that interact with monomeric or filamentous actin and localizes at sites of active actin remodeling, such as lamellipodia and membrane ruffles. Different isoforms of Palladin may have distinct functional roles. It is also implicated in morphological and cytoskeletal changes during dendritic cell maturation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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