Thermo Fisher Scientific Palladin Monoclonal Antibody (1E6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (IHC)

-

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Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Immunocytochemistry (ICC/IF)

1:20-1:100

-

Flow Cytometry (Flow)

5 µg/mL

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Immunoprecipitation (IP)

0.05 mL/2 mL lysate

-

Product Specifications

Species Reactivity

Dog, Chicken, Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1E6

Immunogen

Endogenous Palladin immunoprecipitated from chicken embryo fibroblasts.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

TBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11152174

Product Specific Information

Suggested positive control: MDA-MB231 (human breast cancer) lysates.

Target Information

Palladin is a cytoskeletal protein required for organization of normal actin cytoskeleton. Palladin plays roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. Palladin may function as a scaffolding molecule with the potential to impact both actin polymerization as well as the assembly of existing actin filaments into higher-order arrays. It binds to proteins that bind to either monomeric or filamentous actin. It localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Palladin`s different isoforms may have functional differences. It is also involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.