Thermo Fisher Scientific EIF4E2 Monoclonal Antibody (1A11)
EIF4E2 단백질을 표적하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 1A11). Western blot 및 ELISA에 적합하며, 고순도 친화 크로마토그래피 정제. 인간 단백질 반응성, 액상 형태로 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| ELISA | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A11 |
| Immunogen | EIF4E2 (NP_004837, 146–245 a.a.) partial recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
RCWENLILAM LGEQFMVGEE ICGAVVSVRF QEDIISIWNK TASDQATTAR IRDTLRRVLN LPPNTIMEYK THTDSIKMPG RLGPQRLLFQ NLWKPRLNVP
Target Information
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation (PubMed:9582349, PubMed:17368478, PubMed:25624349).
Acts as a repressor of translation initiation (PubMed:22751931).
In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3), suggesting that it acts by competing with EIF4E and blocking assembly of eIF4F at the cap.
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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