Thermo Fisher Scientific EIF4E2 Monoclonal Antibody (1A11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

ELISA (ELISA)

10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

1A11

Immunogen

EIF4E2 (NP_004837, 146 a.a. approximately 245 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:eIF4E2,uniProtId:O60573-1,ncbiNodeId:9606,antigenRange:146-245,antigenLength:245,antigenImageFileName:H00009470-M02_eIF4E2_O60573-1_House_mouse.svg,antigenImageFileNamePDP:H00009470-M02_eIF4E2_O60573-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: RCWENLILAM LGEQFMVGEE ICGAVVSVRF QEDIISIWNK TASDQATTAR IRDTLRRVLN LPPNTIMEYK THTDSIKMPG RLGPQRLLFQ NLWKPRLNVP

Target Information

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation (PubMed:9582349, PubMed:17368478, PubMed:25624349). Acts as a repressor of translation initiation (PubMed:22751931). In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3), suggesting that it acts by competing with EIF4E and block assembly of eIF4F at the cap. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.