Thermo Fisher Scientific HP1 alpha Recombinant Rabbit Monoclonal Antibody (ARC0244)
HP1 alpha 단백질을 인식하는 재조합 토끼 단클론 항체 ARC0244. Western blot, IHC, ICC/IF, ELISA, IP 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 높은 순도와 안정성, 연구용으로 최적화된 항체.
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Thermo Fisher Scientific HP1 alpha Recombinant Rabbit Monoclonal Antibody (ARC0244)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:800 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0244 |
| Immunogen | Synthetic peptide corresponding to amino acids 92–191 of human HP1 alpha/CBX5 (P45973) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.64 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849298 |
Product Specific Information
Immunogen sequence:
SNFSNSADDI KSKKKREQSN DIARGFERGL EPEKIIGATD SCGDLMFLMK WKDTDEADLV LAKEANVKCP QIVIAFYEER LTWHAYPEDA ENKEKETAKS
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, mediating gene silencing. Mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of adaptor molecules involved in gene silencing and chromatin structure. High-affinity recognition of methylated histone H3 peptide by HP1 requires a functional chromodomain, which serves as a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-knockout mouse fibroblasts but restored after reintroduction of catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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