Thermo Fisher Scientific CHUK Monoclonal Antibody (2G4)
CHUK 단백질을 인식하는 Mouse monoclonal antibody(2G4)로, WB, IHC, ICC/IF, ELISA, PLA 등 다양한 응용에 사용 가능. Human 시료 반응. Affinity chromatography로 정제된 액상 형태이며, PBS(pH 7.4) 버퍼에 보존제 없이 제공. 연구용 전용 시약.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 3 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 10 µg/mL |
| in situ PLA (PLA) | 1:1200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2G4 |
| Immunogen | CHUK (NP_001269, 646–745 a.a.) partial recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
RQKEIWHLLK IACTQSSARS LVGSSLEGAV TPQTSAWLPP TSAEHDHSLS CVVTPQDGET SAQMIEENLN CLGHLSTIIH EANEEQGNSM MNLDWSWLTE
Target Information
CHUK (IKK alpha) is a serine/threonine kinase involved in signal transduction and cellular communication. It belongs to the cytokine-activated protein complex that inhibits the NF-kappa B complex.
IKK alpha, along with IKK beta and the regulatory protein NEMO (IKK gamma), phosphorylates IkB proteins, targeting them for ubiquitin-mediated degradation.
It plays a key role in cellular processes including inflammation and cell survival.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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