Thermo Fisher Scientific CHUK Monoclonal Antibody (2G4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

3 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

ELISA (ELISA)

10 µg/mL

-

in situ PLA (PLA)

1:1200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

2G4

Immunogen

CHUK (NP_001269, 646 a.a. approximately 745 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:IKK alpha,uniProtId:O15111-1,ncbiNodeId:9606,antigenRange:646-745,antigenLength:745,antigenImageFileName:H00001147-M04_IKK_alpha_O15111-1_House_mouse.svg,antigenImageFileNamePDP:H00001147-M04_IKK_alpha_O15111-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: RQKEIWHLLK IACTQSSARS LVGSSLEGAV TPQTSAWLPP TSAEHDHSLS CVVTPQDGET SAQMIEENLN CLGHLSTIIH EANEEQGNSM MNLDWSWLTE

Target Information

CHUK (IKK alpha) is a serine/threonine kinase involved in signal transduction and cellular communication. It belongs to the cytokine activated protein complex that is an inhibitor of the NF-kappa B complex. IKK alpha along with IKK beta and a regulatory protein NEMO (IKK gamma) phosphorylates IkB proteins which targets the later for ubiquitin mediated degradation. It is involved with several cellular processes with extensive studies on its role in inflammation and cell survival.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.