Thermo Fisher Scientific Fc Receptor Binding Inhibitor Polyclonal Antibody, Functional Grade, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

20 µL/test

View 9 publications 9 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

-

Control (Ctrl)

Assay-Dependent

-

Inhibition Assays (IA)

-

View 1 publication 1 publication

Blocking Assay (BLOCK)

-

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Product Specifications

Published species

Human

Class

Polyclonal

Type

Antibody

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

20 µL/Test

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2866336

Product Specific Information

Description: The human FcgammaR-binding inhibitor can be used to inhibit the non-specific Fc-gamma receptor (FcgammaR)-mediated binding of mouse monoclonal antibodies used for flow cytometric analysis of human tissue. Four different classes of Fcgamma receptors have been identified: FcgammaRI (CD64), FcgammaRII (CD32), and FcgammaRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcgammaR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcgammaR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcgammaRs.

Applications Reported: FG Purified Human Fc Receptor Binding Inhibitor has been reported for use in flow cytometric analysis.

To inhibit the non-specific binding of mouse monoclonal antibodies add the human Fc Receptor Binding Inhibitor to samples and incubate on ice for 20 minutes. Without washing proceed to stain with primary antibody.

Applications Tested: FG Purified Human FcgR-Binding Inhibitor has been pre-titrated and tested by inhibiting binding of fluorochrome-conjugated isotype controls to U937 cells. This can be used at 20 µL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

Four different classes of Fcy receptors have been identified: FcyRI (CD64), FcyRII (CD32), and FcyRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcyR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcyR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcyRs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.