Thermo Fisher Scientific Fumarase Polyclonal Antibody
Thermo Fisher Scientific의 Fumarase Polyclonal Antibody는 인간, 마우스, 랫트에 반응하며 Western blot, IHC, ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제된 고품질 항체로, 4°C 또는 -20°C에서 안정적으로 보관 가능합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 195–473 of fumarate hydratase (Uniprot ID: P07954) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.49 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11153711 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse liver, rat liver
- Predicted reactivity: Rat (98%), Zebrafish (90%), Pig (98%), Chicken (91%), Sheep (99%), Chimpanzee (99%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The fumarase protein is an enzymatic component of the tricarboxylic acid (Krebs) cycle, catalyzing the formation of L-malate from fumarate. It exists in both cytosolic and mitochondrial forms, differing in translation start site. The mitochondrial form results from removal of an N-terminal extension. This enzyme functions as a homotetramer and is similar to thermostable class II fumarases. Mutations in this gene can cause fumarase deficiency, leading to progressive encephalopathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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