Thermo Fisher Scientific ID3 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Product Specifications

Product Specific Information

Antibody detects endogenous levels of total ID3.

Target Information

Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3, and ID4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA, thus negatively regulating DNA binding of bHLH proteins.

ID1 inhibits DNA binding of E12 and Myo D, functioning to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, ID protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which activate myogenic differentiation. Expression of each ID protein is strongly dependent on growth factor activation, and reduction of ID mRNA levels by antisense oligonucleotides delays reentry of arrested cells into the cell cycle following growth factor stimulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.