Thermo Fisher Scientific ID3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ID3(Accession Q02535), corresponding to amino acid residues S65-R115. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ID3,uniProtId:Q02535-1,ncbiNodeId:9606,antigenRange:65-115,antigenLength:119,antigenImageFileName:PA5-100197_ID3_Q02535-1_Rabbit.svg,antigenImageFileNamePDP:PA5-100197_ID3_Q02535-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2815727

Product Specific Information

Antibody detects endogenous levels of total ID3.

Target Information

Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3 and ID4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The ID-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since ID1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the ID protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the ID proteins is strongly dependent on growth factor activation and that reduction of ID mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.