Thermo Fisher Scientific Wilm`s Tumor 1 (WT1) (Wilm`s Tumor and Mesothelial Marker) Monoclonal Antibody (WT1/7451)
WT1 단백질을 표적으로 하는 Thermo Fisher Scientific의 단클론 항체로, 인간 시료에 반응하며 Western blot, IHC, ELISA 등에 사용 가능. 단백질 A/G로 정제된 액상 형태이며, PBS buffer에 보존제 없이 제공됨. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | WT1/7451 |
| Immunogen | Recombinant fragment (around aa150–350) of human WT1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Target Information
The Wilms Tumor 1 gene encodes one of the WPP domain-interacting proteins required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in nucleocytoplasmic transport, spindle formation, and nuclear envelope reassembly. The cytoplasmic RanGAP is critical for establishing a functional RanGTP/RanGDP gradient across the nuclear envelope and is associated with its outer surface in metazoan and higher plant cells. Arabidopsis thaliana RanGAP association requires nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). Wilms Tumor 1 and WIT2 form a second family of CC-TMD proteins necessary for RanGAP nuclear envelope association in root tip cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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