Thermo Fisher Scientific Wilm`s Tumor 1 (WT1) (Wilm`s Tumor and Mesothelial Marker) Monoclonal Antibody (WT1/7452)
Human WT1 단백질을 인식하는 마우스 단클론 항체로, IHC(P), IHC(PFA), ELISA에 사용 가능. Recombinant WT1(aa150-350) 항원으로 제작됨. 단백질 A/G로 정제된 액상형, 1 mg/mL 농도. -20°C 보관, 연구용 전용.
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Applications
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | WT1/7452 |
| Immunogen | Recombinant fragment (around aa150–350) of human WT1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Target Information
The Wilms Tumor 1 (WT1) gene encodes one of the WPP domain-interacting proteins required for RanGAP nuclear envelope association in root tip cells. Ran GTPase is essential for multiple cellular processes, including nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly. The cytoplasmic Ran GTPase activating protein RanGAP is critical to establish a functional RanGTP/RanGDP gradient across the nuclear envelope. In Arabidopsis thaliana, RanGAP association with the root tip nuclear envelope requires nucleoporins combining coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). Wilms Tumor 1 and WIT2 are a second family of CC-TMD proteins required for RanGAP nuclear envelope association in root tip cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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