Thermo Fisher Scientific Wilm`s Tumor 1 (WT1) (Wilm`s Tumor and Mesothelial Marker) Monoclonal Antibody (6F-H2)
WT1 단백질을 인식하는 단클론 항체로, 인간·마우스·랫트 조직에서 IHC(P) 및 IHC(PFA) 분석에 사용 가능. Recombinant WT1(1–181) 항원을 기반으로 제작되었으며, Protein A/G 정제, PBS 기반 저장 버퍼 포함. 연구용으로만 사용.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: Assay-dependent
Immunohistochemistry (PFA fixed) (IHC (PFA))
- Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6F-H2 |
| Immunogen | Recombinant protein corresponding to residues 1–181 of human WT1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C, do not freeze |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Target Information
The Wilms Tumor 1 (WT1) gene encodes one of the WPP domain-interacting proteins required for RanGAP nuclear envelope association in root tip cells. Ran GTPase plays essential roles in nucleocytoplasmic transport, spindle formation, and postmitotic nuclear envelope reassembly. RanGAP is critical for establishing a functional RanGTP/RanGDP gradient across the nuclear envelope and is associated with the outer surface of the nuclear envelope in metazoan and higher plant cells. Arabidopsis thaliana RanGAP association with the root tip nuclear envelope requires plant-specific nucleoporins with coiled-coil and transmembrane domains (CC-TMD) and WPP domain-interacting proteins (WIPs). WT1 and WIT2 form a second family of CC-TMD proteins that are structurally similar but distinct from WIPs, essential for RanGAP nuclear envelope association.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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