Thermo Fisher Scientific DMPO Monoclonal Antibody (N1664A), PE
DMPO 단백질 및 DNA 라디칼 검출용 마우스 모노클로날 항체. PE 형광 표지로 다양한 면역분석(WB, IHC, ELISA 등)에 사용 가능. 높은 특이성과 재현성을 제공하며, 단백질 G 정제 및 보존제 무첨가. 연구용으로만 사용.
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Thermo Fisher Scientific DMPO Monoclonal Antibody (N1664A), PE
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | 1:100 |
| Immunoprecipitation (IP) | 25 µg |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N1664A |
| Immunogen | 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin |
| Conjugate | PE |
| Excitation/Emission Max | 565/576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931718 |
Available Formats
- Unconjugated (MA5-27550)
- APC (MA5-45261)
- FITC (MA5-45262)
- PerCP (MA5-45263)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45264 was sufficient to detect DMPO nitrone adducts of metmyoglobin (100 ng/lane) by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts, and DMPO-DNA adducts.
Does not cross-react with non-adducted proteins or DNA.
Target Information
The formation of free radicals and other highly reactive oxygen species is implicated in many disease states. Identifying these species is crucial, and spin trapping has enabled this detection.
DMPO (5,5-dimethyl-1-pyrroline N-oxide) is relatively non-toxic and exhibits favorable pharmacokinetics in biological systems.
Studies show that nitric oxide can affect DMPO adduct quantification, requiring caution when studying species generation in its presence.
DMPO adducts can form with protein and DNA radicals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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