Thermo Fisher Scientific DMPO Monoclonal Antibody (N1664A), FITC
DMPO 단백질 및 DNA 아디덕트 검출용 FITC 결합 단클론 항체. 다양한 응용(WB, IHC, ICC, ELISA, IP)에 사용 가능. 1 mg/mL 농도의 액상 형태로 제공되며, 4°C 암소 보관. Protein G로 정제된 Mouse IgG1 항체.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | 1:100 |
| Immunoprecipitation (IP) | 25 µg |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N1664A |
| Immunogen | 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 581 mM DMSO, 123 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931716 |
Available Formats
- Unconjugated (MA5-27550)
- APC (MA5-45261)
- PE (MA5-45264)
- PerCP (MA5-45263)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45262 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts, and DMPO-DNA adducts.
Does not cross-react with non-adducted proteins or DNA.
Target Information
The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. Spin trapping enables identification of these species.
DMPO (5,5-dimethyl-1-pyrroline N-oxide) is a low-toxicity spin trap with favorable pharmacokinetics in biological systems.
Nitric oxide may interfere with DMPO adduct quantification; caution is required when studying species generation in the presence of nitric oxide or its radicals.
DMPO adducts can be generated with protein and DNA radicals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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