Thermo Fisher Scientific DMPO Monoclonal Antibody (N1664A), APC
DMPO 단백질 및 DNA 아닥트를 특이적으로 인식하는 Thermo Fisher Scientific의 DMPO Monoclonal Antibody (N1664A), APC 형광표지 항체. 다양한 응용(ELISA, WB, IHC 등)에 사용 가능하며, 높은 특이성과 재현성을 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | 1:100 |
| Immunoprecipitation (IP) | 25 µg |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N1664A |
| Immunogen | 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931715 |
Available Formats
- Unconjugated (MA5-27550)
- FITC (MA5-45262)
- PE (MA5-45264)
- PerCP (MA5-45263)
- Request custom conjugation (Form Link)
Product Specific Information
A 1:1,000 dilution of MA5-45261 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts, and DMPO-DNA adducts.
Does not cross-react with non-adducted proteins or DNA.
Target Information
The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal.
DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic spin traps to cells and animals, with convenient pharmacokinetics in biological systems.
Nitric oxide may affect the quantitative determination of DMPO adducts; caution is advised when studying these species in the presence of nitric oxide or its radicals.
DMPO adducts can be generated with protein and DNA radicals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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