Thermo Fisher Scientific HSP701L Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:10,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 335 and 639 of Human HSP70 1L if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HSP701L,uniProtId:P34931-1,ncbiNodeId:9606,antigenRange:335-639,antigenLength:641,antigenImageFileName:PA5-28202_HSP701L_P34931-1_Rabbit.svg,antigenImageFileNamePDP:PA5-28202_HSP701L_P34931-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2545678

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse testis, rat testis, U87-MG, SK-N-SH, IMR32, SK-N-AS.

Predicted reactivity: Mouse (93%), Rat (92%), Xenopus laevis (81%), Pig (94%), Bovine (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock proteins. One class of stress proteins, termed the small heat shock proteins is comprised of a diverse group of proteins from approximately15 to >30 kDa, with a single form found in yeast and human cells and multiple related forms found in higher plants. A direct relationship between elevated strwess protein expression and neoplasia has been suggested from studies on estrogen-induced proteins in breast cancer cells. In addition, Hsp27 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoter (e. g. phorbol esters) and calcium ionophores. The expression of a 24 kDa protein was shown to correlate with steroid hormone receptor in cell lines, organs, and tumors. The 24 kDa estrogen-induced protein was found to be expressed at high levels in carcinoma of the breast. Another study has suggested that levels of the Hsp27 correlate with endometrial adenocarcinomas. Interestingly, the regulation of human Hsp27 by estrogens and heat shock is reminiscent of the ecdysteroid and heat shock activation of the drosophila Hsp20 genes.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.