Thermo Fisher Scientific Midnolin Polyclonal Antibody
Midnolin 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. Protein A 정제, 1 mg/mL 농도. -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human MIDN, amino acids 361–468 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Expression of the midnolin gene is developmentally regulated: it is strongly expressed in the mesencephalon (midbrain) of embryos at day 12.5 (E12.5) in mice. The midnolin gene encodes a protein of 508 amino acids containing a ubiquitin-like domain. Intracellular localization studies using midnolin-GFP fusion proteins showed that midnolin is localized in the nucleus and nucleolus, but not in the cytoplasm. The nucleolar localization signal is a 28-amino-acid peptide located at the C-terminal region of the protein. Midnolin may be involved in the regulation of genes related to neurogenesis in the nucleolus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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