Thermo Fisher Scientific IL-12 alpha Polyclonal Antibody
다양한 응용(WB, IHC, ICC, Flow, ELISA)에 사용 가능한 IL-12 alpha 폴리클로날 항체. Mouse, Rat 반응성. Rabbit IgG 기반, Protein A 정제. 고농도(1 mg/mL) 액상 형태로 -20°C 보관. 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from mouse IL-12 alpha, amino acids 51–150 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Interleukin 12A (IL-12 alpha) is a signaling cytokine belonging to the Interleukin-6 protein superfamily. The IL-12A precursor is a 35 kDa subunit of the disulfide-linked heterodimeric cytokine IL-12, exhibiting diverse biological activities. IL-12A functions as a growth factor for activated T and NK cells, enhancing NK/lymphokine-activated killer cell lytic activity. It stimulates IFN-gamma production by PBMCs and promotes differentiation of Th1 and Th2 cells. IL-12A also regulates tyrosine phosphorylation of Stat4 and is involved in innate immune signaling requiring nitric oxide synthase 2A. Together with EBI3, IL-12A forms interleukin-35 (IL-35), a heterodimeric cytokine produced by Treg cells essential for maximal suppressive activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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