Thermo Fisher Scientific Rat anti-Mouse IgG2a Secondary Antibody, Super Bright 780, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 1.0 µg/test

Product Specifications

Additional Available Conjugates

• APC
• Biotin
• eFluor 570
• FITC
• HRP
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Custom conjugation available

Product Specific Information

The monoclonal antibody m2a-15F8 recognizes mouse IgG2a antibodies and is used as a secondary reagent in flow cytometry and microscopy. It does not cross-react with other mouse isotypes or any rat immunoglobulins.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested by flow cytometry using normal human peripheral blood cells
• Recommended ≤1.0 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Optimize titration for best performance

Super Bright 780 is a tandem dye excited by the violet laser (405 nm) and emits at 780 nm. Use a 780/60 bandpass filter and ensure instrument compatibility.

Experimental Notes

• Compensation values may differ when using UltraComp eBeads (Product #01-2222-42) or AbC Total Antibody Compensation Beads (Product #A10497).
• When using multiple Super Bright conjugates, use Super Bright Complete Staining Buffer (Product #SB-4401) to minimize non-specific interactions.
• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C.

Excitation: 405 nm
Emission: 780 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Anti-Mouse secondary antibodies are affinity-purified with high specificity for mouse immunoglobulins. These reagents are useful for detection, sorting, or purification of target antibodies. Secondary antibodies enhance sensitivity and versatility through various detection systems such as HRP, AP, or fluorescence. They are typically produced by immunizing the host with pooled mouse immunoglobulins and can be further purified or modified for high specificity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지