Thermo Fisher Scientific MDM2 Polyclonal Antibody
MDM2 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 적합합니다. 고순도(>95%) 항체로 p53 조절 연구에 유용하며, Human, Mouse, Rat 시료에 반응합니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- PA586249
- 판매단위
- pk
카탈로그
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | – |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | – |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 130–180 of Human MDM2 (UniProt ID: Q00987-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2803038 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
MDM2 is a ubiquitin ligase for p53 and plays a central role in regulating p53 stability. It is located on chromosome 12q. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 enhances its interaction with p300, promoting MDM2-mediated ubiquitination and degradation of p53. Phosphorylation also inhibits binding to p19ARF, further increasing p53 degradation. Overexpression of MDM2 can inactivate p53 and reduce its tumor suppressor function. MDM2 also has E3 ubiquitin ligase activity and is involved in apoptosis and cell cycle regulation. It is overexpressed in various human cancers, including soft tissue carcinomas and breast cancer. Additionally, MDM2 interacts with proteins such as retinoblastoma 1 and ribosomal protein L5, and more than 40 alternatively spliced transcript variants have been identified in tumor and normal tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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