Thermo Fisher Scientific SSEA5 Monoclonal Antibody (8E11), DyLight 650
DyLight 650 형광 표지된 SSEA5 단클론 항체로 인간 배아줄기세포 및 유도만능줄기세포의 미분화 상태 검출에 적합. IHC, ICC, Flow Cytometry에 사용 가능. 높은 특이성과 안정된 신호 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:50–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8E11 |
| Immunogen | Undifferentiated H9 human embryonic stem cells |
| Conjugate | DyLight™ 650 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C, do not freeze |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536851 |
Additional Formats
Product Specific Information
Flow cytometric analysis of MA1-144-D650 shows positive staining of SSEA-5 in human embryonal carcinoma NCCIT cells.
Target Information
Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide component of glycosphingolipids and glycoproteins. It contains the motif Fuc1-2Gal-β1-3GlcNAc-β, characteristic of H-1 antigens.
SSEA-5 is expressed in the inner cell mass of the human blastocyst, on undifferentiated human embryonic stem cells, and on the surface of induced pluripotent stem cells.
It precedes the loss of other stem cell markers, and combinations of SSEA-5 with CD9/CD90 or CD50/CD200 improve identification and elimination of undifferentiated human ES cells compared to other established markers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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