Thermo Fisher Scientific SSEA5 Monoclonal Antibody (8E11), DyLight 650

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

1:50

-

Immunocytochemistry (ICC/IF)

1:50

-

Flow Cytometry (Flow)

1:50-1:500

-

Product Specifications

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

8E11

Immunogen

Undifferentiated H9 human embryonic stem cells

Conjugate

DyLight™ 650 DyLight™ 650 DyLight™ 650

View additional formats

• Unconjugated
• DyLight 488
• PE
• Request custom conjugation

Excitation/Emission Max

651/673 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536851

Product Specific Information

Flow cytometric analysis of MA1-144-D650 shows positive staining of SSEA-5 in human embryonal carcinoma NCCIT cells.

Target Information

Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide that can be part of glycosphingolipids and glycoproteins. It contains the motif Fuc1-2Gal-beta1-3GlcNAc-beta, which is characteristic of H-1 antigens. SSEA-5 is found in the inner cell mass of the human blastocyst during embryogenesis, on undifferentiated human embryonic stem cells and on the surface of induced pluripotent cells. SSEA-5 was found to proceed the loss of other known stem cell markers and the combination of SSEA-5 with either CD9 and CD90 or CD50 and CD200 was superior in identifying and eliminating rare undifferentiated human ES cells from differentiated cell populations than the use of other established stem cell markers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.