Thermo Fisher Scientific NuMA Polyclonal Antibody
NuMA 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC 등 다양한 응용에 사용 가능. Human에 반응하며 Mouse, Rat, Bovine에 예측 반응성 있음. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:10,000 | View 2 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 1–339 of NuMA (Uniprot ID: Q14980) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.54 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at −20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11157241 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, HeLa (100 ng/mL Nocodazole treatment for 24 hr)
- Predicted reactivity: Mouse (81%), Rat (83%), Bovine (89%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
NuMA (nuclear mitotic apparatus) is a long coiled-coil protein involved in both interphase and mitosis.
During interphase, it may play a structural role in the nucleoskeleton, contributing to nuclear organization and function.
During mitosis, it is associated with spindle microtubule organization and chromosome positioning.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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