Thermo Fisher Scientific Bcl-2 Polyclonal Antibody
Bcl-2 단백질을 표적하는 Rabbit Polyclonal Antibody로, 세포 사멸(apoptosis) 연구에 적합합니다. WB, IHC, ICC/IF 등 다양한 응용에 사용 가능하며, 고순도 항원 친화 크로마토그래피로 정제되었습니다. -20°C에서 보관하며 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–50 µg/mL |
| Immunocytochemistry (ICC/IF) | 5–50 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein B-Cell Leukemia/Lymphoma 2 (BCL-2). The antigen corresponds to amino acid range 2–208 of the target protein. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2903477 |
Target Information
BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. Members of the BCL-2 family can form dimers to modulate apoptosis. For example, Bag1 forms a heterodimer with BCL-2, enhancing its anti-apoptotic effect. Bax and Bak are critical for cytochrome c release from mitochondria, initiating apoptosis. Constitutive expression of BCL-2, such as translocation to the Ig heavy chain locus, is associated with follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas normal germinal centers are negative. Two transcript variants of BCL-2 differ at their C-terminal ends. Overexpression of BCL-2 has been linked to cancers such as B-cell lymphoma and prostate cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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