Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (29A1.4), APC-eFluor 780, eBioscience

Thermo Fisher Scientific CD335 (NKp46) Monoclonal Antibody (29A1.4), APC-eFluor 780

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1 µg/test
• View 17 publications

Product Specifications

Additional Formats

• Alexa Fluor 700
• APC
• Biotin
• Brilliant Violet 711
• eFluor 450
• eFluor 660
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The monoclonal antibody 29A1.4 recognizes mouse NKp46 (CD335), a member of the natural cytotoxicity receptor (NCR) family.
CD335 is a glycoprotein with two Ig-like domains and a short cytoplasmic tail, expressed uniquely on NK cells, including immature NK cells (DX5-, CD3-), enabling discrimination between NKT and NK cells (NKp46+, CD3-).
Staining is not strain-specific and has been shown on C57Bl/6, SJL, CBA/CA, and BALB/C strains. NKp46 plays a role in NK cell-mediated lysis of tumor and pathogen-infected cells.

• Activation: The 29A1.4 monoclonal antibody activates NK cells in vitro but does not deplete NK cells in vivo.
• Applications Reported: Flow cytometric analysis.
• Applications Tested: Tested on mouse spleen cells, ≤1 µg/test (100 µL final volume).
  • Recommended to titrate for optimal performance.
  • Cell number: 10⁵–10⁸ cells/test.

Fluorophore Information:

• APC-eFluor® 780 emits at 780 nm; excitation at 633 nm (Red laser).
• Ensure instrument compatibility.
• Light sensitive—protect from light.
• Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in dark with minimal impact on brightness or FRET efficiency.
• Excitation: 633–647 nm
• Emission: 780 nm
• Laser: Red
• Filtration: 0.2 µm post-manufacturing filtered.

Target Information

NKp46 (CD335, NCR1) is a cytotoxicity-activating receptor contributing to NK cell-mediated tumor cell lysis.
It consists of two Ig-like domains related to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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