Thermo Fisher Scientific CCDC134 Polyclonal Antibody
인간 CCDC134 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, WB, ELISA, IP에 사용 가능. 보존제 무첨가, 단백질 A 및 항원 친화 크로마토그래피로 정제됨. 연구용으로 MAPK-매개 Elk1 전사 조절 연구에 적합.
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Thermo Fisher Scientific CCDC134 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:25,000–1:50,000 |
| Immunoprecipitation (IP) | 0.2–1 µL/mg of lysate |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human CCDC134 protein (Met1–Leu229) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787731 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human CCDC134.
Target Information
The coiled-coil domain is a common protein motif involved in protein oligomerization and is found in proteins such as transcription factors and intermediate filaments.
CCDC134 is a secretory protein that inhibits the transcriptional activity of Elk1. Overexpression of CCDC134 inhibits phosphorylation of Erk and JNK/SAPK but not p38 MAPK. Conversely, siRNA against CCDC134 activates Elk1 transcriptional activity and phosphorylation of Erk and JNK/SAPK, suggesting an inhibitory role of CCDC134 in MAPK-mediated Elk1 transcription.
CCDC134 is widely expressed in normal adult tissues, tumors, and cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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