Thermo Fisher Scientific CEP135 Polyclonal Antibody
CEP135 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA 등 다양한 응용에 적합합니다. Human, Mouse, Rat에 반응하며, Protein A 정제 및 고순도 액상 형태로 제공됩니다. -20°C에서 보관하며 연구용으로 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human CEP135 (amino acids 1001–1100) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
LATS1 is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with the cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle-dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. The C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor, supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors, and high sensitivity to carcinogenic treatments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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