Thermo Fisher Scientific Leptin Monoclonal Antibody (3)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

1:40,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3

Immunogen

Purified, recombinant Leptin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Leptin,uniProtId:P41159-1,ncbiNodeId:9606,antigenRange:1-167,antigenLength:167,antigenImageFileName:ABS_022-03-02_Leptin_P41159-1_House_mouse.svg,antigenImageFileNamePDP:ABS_022-03-02_Leptin_P41159-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.09 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_667480

Product Specific Information

ABS 022-03-02 detects Leptin in human samples.

ABS 022-03-02 has been successfully used in ELISA and immunohistochemistry procedures. ABS 022-03-02 binds free Leptin in solution. In sandwich ELISA, ABS 022-03-02 works as a capture antibody with ABS 022-11-02 as a detection antibody.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin`s effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure. Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors. Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth. Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.