Thermo Fisher Scientific ACTL6A Monoclonal Antibody (3E4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

ELISA (ELISA)

10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3E4

Immunogen

ACTL6A (AAH00949, 1 a.a. approximately 387 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BAF53A,uniProtId:O96019-1,ncbiNodeId:9606,antigenRange:1-429,antigenLength:429,antigenImageFileName:H00000086-M14_BAF53A_O96019-1_House_mouse.svg,antigenImageFileNamePDP:H00000086-M14_BAF53A_O96019-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: MVVERDDGST LMEIDGDKGK QGGPTYYIDT NALRVPRENM EAISPLKNGM VEDWDSFQAI LDHTYKMHVK SEASLHPVLM SEAPWNTRAK REKLTELMFE HYNIPAFFLC KTAVLTAFAN GRSTGLILDS GATHTTAIPV HDGYVLQQGI VKSPLAGDFI TMQCRELFQE MNIELVPPYM IASKEAVREG SPANWKRKEK LPQVTRSWHN YMCNCVIQDF QASVLQVSDS TYDEQVAAQM PTVHYEFPNG YNCDFGAERL KIPEGLFDPS NVKGLSGNTM LGVSHVVTTS VGMCDIDIRP GLYGSVIVAG GNTLIQSFTD RLNRELSQKT PPSMRLKLIA NNTTVERRFS SWIGGSILAS LGTFQQMWIS KQEYEEGGKQ CVERKCP

Target Information

BAF53A is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.