Thermo Fisher Scientific PCNA Monoclonal Antibody (UMAB278), UltraMAB

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:4,000

Product Specifications

Product Specific Information

• For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
• For conjugation experiments, perform an additional round of desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and repair. It interacts with CDK inhibitor p21, Fen1, XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA serves as a marker for proliferating cells and is useful for assessing tumor proliferation. It is localized in the nucleus, functions as a homotrimer, and enhances leading strand synthesis during DNA replication.
Upon DNA damage, PCNA is ubiquitinated and participates in the RAD6-dependent repair pathway. Two transcript variants encoding the same protein are known, with pseudogenes identified on chromosome 4 and X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.