Thermo Fisher Scientific MAP3K8 Monoclonal Antibody (OTI2E10), TrueMAB

Applications

• Western Blot (WB): 1:2,000 dilution tested

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

MAP3K8 (COT) is a serine/threonine kinase that activates both the MAP and JNK kinase pathways and plays a critical role in cell cycle regulation.
The MAPK signaling cascade involves sequential activation of MAPKKK (MEKK), MAPKK (MEK), and MAPK (ERK). These kinases are highly conserved across species including yeast, Drosophila, and mammals.
MEKK8 activates NF-kappa-B1 by promoting proteasome-mediated proteolysis of NF-kappa-B1/p105. This cytoplasmic protein is expressed in various normal tissues and tumor-derived cell lines.
The 58 kDa form is specifically activated during the S and G2/M phases of the cell cycle, with phosphorylation occurring mainly on serine residues in the longer form and on both serine and threonine residues in the shorter form.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.