Thermo Fisher Scientific Phospho-IRS1 (Ser636) Polyclonal Antibody
인간 및 생쥐에서 phospho-IRS1 (Ser636)을 검출하는 Rabbit Polyclonal 항체. Western blot에 최적화되어 있으며, 항원 친화 크로마토그래피로 정제됨. PBS/BSA 버퍼에 보관되며 -20°C에서 저장. 인슐린 신호전달 관련 연구에 적합.
- 카탈로그번호
- PA11055
- 판매단위
- pk
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Thermo Fisher Scientific Phospho-IRS1 (Ser636) Polyclonal Antibody
Applications
- Western Blot (WB)
Tested Dilution: 2 µg/mL
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide corresponding to residues D(631) Y M P M (pS) P K S V S A P Q Q I(646) of human IRS-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice (domestic); Dry ice (international) |
| RRID | AB_325023 |
Product Specific Information
PA1-1055 detects phospho-IRS-1 (Ser636) in mouse and human cells.
This antibody has been successfully used in Western blot procedures.
By Western blot, it detects a ~170 kDa phospho-IRS-1 (Ser636) protein from 3T3-L1 cells treated with insulin for 10 minutes.
The immunogen is a synthetic phosphopeptide corresponding to residues D(631) Y M P M (pS) P K S V S A P Q Q I(646) of human IRS-1.
This peptide sequence is 100% conserved in rats and mice.
The peptide (Cat. # PEP-185) is available for neutralization and control experiments.
Target Information
IRS-1 is a major substrate of the insulin receptor and is phosphorylated in response to stimulation by insulin, IGF-1, and IL-4.
Phosphorylation occurs on serine, threonine, and tyrosine residues in various tissues.
Casein kinase II mediates part of the insulin-stimulated serine/threonine phosphorylation of IRS-1 in vivo.
Thr502 and Ser99 are identified as major phosphorylation sites catalyzed by casein kinase II.
IRS-1 contains three putative 14-3-3 binding sites (Ser270, Ser374, Ser641), with the motif around Ser270 located in the phosphotyrosine-binding domain responsible for insulin receptor interaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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