Thermo Fisher Scientific IRS1 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• PA1-1058 detects IRS-1 in mouse and human cells.
• Successfully used in Western blot procedures.
• Detects an approximately 170 kDa protein representing IRS-1 in 3T3 L1 cell extract.
• Immunogen: synthetic peptide corresponding to residues L(602)HTDDGYMPMSPGV(615) of human IRS-1.
• The immunizing peptide is 92% conserved in rats and mice.
• This peptide (Cat. # PEP-192) is available for use in neutralization and control experiments.

Target Information

IRS-1 (Insulin Receptor Substrate 1) is a major substrate of the insulin receptor, phosphorylated in response to insulin, IGF-1, and IL-4 stimulation. It is phosphorylated on serine, threonine, and tyrosine residues across various tissues. Casein kinase II mediates part of the insulin-stimulated phosphorylation, with Thr502 and Ser99 identified as key phosphorylation sites. IRS-1 contains three putative 14-3-3 binding sites (Ser270, Ser374, Ser641), with Ser270 located within the phosphotyrosine binding domain responsible for interaction with the insulin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.