Thermo Fisher Scientific TK1 Recombinant Rabbit Monoclonal Antibody (JF0970)
TK1 단백질을 인식하는 재조합 토끼 단클론 항체로, 높은 특이성과 민감도를 제공. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. Lot 간 일관성 유지 및 동물 유래 성분 없음. 연구용으로 장기 안정적 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF0970 |
| Immunogen | Synthetic peptide within Human Thymidine Kinase 1 (aa 2–49) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2809790 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Thymidine Kinase (TK1) is a conserved phosphotransferase present in most living cells. It catalyzes the reaction:
deoxythymidine + ATP → deoxythymidine 5'-phosphate + ADP,
which is crucial for incorporating deoxythymidine into DNA. TK1 is essential for the activity of antiviral drugs such as azidothymidine (AZT) and is used for hybridoma selection in monoclonal antibody production. TK1 expression correlates with cell division, making it clinically relevant in proliferative studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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