Thermo Fisher Scientific NEDD8 Polyclonal Antibody
NEDD8 단백질을 인식하는 Thermo Fisher Scientific의 sheep polyclonal antibody. Western blot 및 immunohistochemistry(Frozen/Paraffin)에서 사용 가능. 인간, 마우스, 랫트 반응성. 동결건조 형태로 제공되며 장기 보관 시 -20°C 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:300–1:2,000 | - |
| Immunohistochemistry (Frozen) (IHC-F) | 1:300–1:2,000 | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Sheep / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide from the N-terminal of mouse NEDD8 conjugated to blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The antigen is homologous in human, rat, zebrafish, drosophila, and xenopus.
- Specificity: NEDD8.
Target Information
NEDD8 is a ubiquitin-like protein involved in cell cycle control and embryogenesis. Covalent attachment to substrates requires activation by the E1 complex (UBE1C-APPBP1) and transfer via the E2 enzyme (UBE2M). NEDD8 attachment to cullins activates their associated E3 ubiquitin ligase activity, promoting polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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