
Thermo Fisher Scientific NEDD8 Polyclonal Antibody
NEDD8 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 Immunohistochemistry에 적합합니다. 사람, 마우스, 랫트 반응성이 있으며 동결건조 형태로 제공됩니다. 세포 주기 조절 및 단백질 분해 관련 연구에 활용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:300–1:2,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:300–1:2,000 | - |
| Miscellaneous (PubMed) | - | View 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from the N-terminal of mouse NEDD8 (Ubiquitin-like protein Nedd8, Neddylin) conjugated to an immunogenic carrier protein. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The peptide is homologous in human, rat, zebrafish, drosophila, and xenopus.
- Specificity: NEDD8.
Target Information
NEDD8 is a ubiquitin-like protein that plays an important role in cell cycle control and embryogenesis.
Covalent attachment to its substrates requires activation by the E1 complex (UBE1C-APPBP1) and linkage to the E2 enzyme (UBE2M).
Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, promoting polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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