Thermo Fisher Scientific AHA1 Monoclonal Antibody (4H9-D7), PerCP
AHA1 단백질을 인식하는 PerCP 결합 마우스 단일클론 항체. Western blot, ICC/IF, ELISA에 적합. HSP90AA1의 보조 샤페론 연구에 활용 가능. 사람 및 생쥐 Aha1/Aha2 검출에 유용. 연구용 전용 시약.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4H9-D7 |
| Immunogen | Human Aha1, His-tagged |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
1 µg/mL of MA5-45290 was sufficient for detection of Aha1 in 10 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 38 kDa, can run up to 45 kDa on SDS-PAGE. Recognizes human Aha1, mouse Aha1, and mouse Aha2.
Target Information
Acts as a co-chaperone of HSP90AA1. Activates the ATPase activity of HSP90AA1, increasing its chaperone activity. Competes with inhibitory co-chaperones FNIP1 and TSC1 for binding to HSP90AA1, providing a reciprocal regulatory mechanism for chaperoning of client proteins. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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