Thermo Fisher Scientific DNMT1 Monoclonal Antibody (60B1220.1), APC
DNMT1 단백질을 검출하기 위한 Thermo Fisher Scientific의 APC 결합 단클론 항체. Western blot, IHC, IP, ChIP 등 다양한 응용에 적합. 인간, 생쥐, 어류, 제브라피시 반응성. 고순도 Protein G 정제, 4°C 보관.
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Thermo Fisher Scientific DNMT1 Monoclonal Antibody (60B1220.1), APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:10,000 |
| Immunoprecipitation (IP) | Assay-dependent |
| ChIP assay (ChIP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Fish, Human, Mouse, Zebrafish |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 60B1220.1 |
| Immunogen | Synthetic peptide corresponding to amino acids 637–650 of human DNMT1 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931745 |
Available Formats
- FITC ([MA5-45292])
- PerCP ([MA5-45293])
- Custom conjugation available upon request
Product Specific Information
- 2 µg/mL of MA5-45291 detects Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot using Goat anti-mouse IgG:HRP.
- Detects approximately 180 kDa.
- Cross-reacts with mouse DNMT1.
Target Information
This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for cytokinesis completion. The encoded protein interacts with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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