Thermo Fisher Scientific Phospho-BTK/ITK (Tyr551, Tyr511) Monoclonal Antibody (M4G3LN), eFluor 660, eBioscience
BTK 및 ITK의 인산화 형태(Tyr551, Tyr511)를 인식하는 마우스 단클론 항체로, 인체 및 마우스 시료에 모두 반응합니다. eFluor 660 형광 표지로 IHC 및 ICC/IF 분석에 적합하며, 4°C 암소 보관이 필요합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M4G3LN |
| Conjugate | eFluor™ 660 |
| Excitation/Emission Max | 651/668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574307 |
Additional Formats
Product Specific Information
This M4G3LN monoclonal antibody recognizes human and mouse Bruton’s tyrosine kinase (BTK) and interleukin-2-inducible tyrosine kinase (ITK) when phosphorylated on Y551 and Y511, respectively.
Tec family kinases, including BTK and ITK, are critical in B and T cell receptor signaling and activation of calcium-dependent pathways.
Deficiencies in BTK or ITK result in impaired B or T cell development, leading to immunodeficiency disorders such as X-linked agammaglobulinemia.
Specificity of this clone was validated by ELISA, flow cytometry, and western blotting.
Applications Reported:
- Immunohistochemical staining (IHC)
- IHC of formalin-fixed paraffin-embedded tissue sections
Applications Tested:
- Tested by IHC on FFPE human tissue using high pH antigen retrieval
- Recommended ≤10 µg/mL (optimize by titration)
Fluorochrome Information:
eFluor™ 660 replaces Alexa Fluor™ 647.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Tec family kinases (BTK, ITK, Tec, Rlk) are receptor-proximal kinases essential for activation of phospholipase C and calcium signaling following antigen receptor stimulation.
BTK mediates signaling downstream of LPS-TLR4 in B cells via RP105/CD180.
Mice lacking BTK or ITK show severe defects in lymphocyte signaling, and human BTK mutations cause X-linked agammaglobulinemia and immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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