Thermo Fisher Scientific TRAIL (soluble) Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sTRAIL/Apo2L. Anti-Human sTRAIL/Apo2L-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human sTRAIL/Apo2L by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sTRAIL/Apo2L (500-P135) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human sTRAIL/Apo2L.

Western Blot:
To detect Human sTRAIL/Apo2L by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Human sTRAIL/Apo2L is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P135BT-1MG will be provided as 2 × 500 µg.

Target Information

TRAIL is a cytokine belonging to the tumor necrosis factor (TNF) ligand family. It preferentially induces apoptosis in transformed and tumor cells but does not appear to kill normal cells, although expressed in most normal tissues.
TRAIL binds to several members of the TNF receptor superfamily, including:

• TNFRSF10A/TRAILR1
• TNFRSF10B/TRAILR2
• TNFRSF10C/TRAILR3
• TNFRSF10D/TRAILR4
• TNFRSF11B/OPG

The activity of TRAIL may be modulated by binding to decoy receptors (TRAILR3, TRAILR4, OPG) that cannot induce apoptosis.
Binding of TRAIL to its receptors triggers activation of MAPK8/JNK, caspase 8, and caspase 3.
Receptors include death receptor 4 (DR4) and death receptor 5 (DR5), as well as decoy receptors DcR1 and DcR2.
TRAIL is a type II membrane protein expressed in various human tissues and induces apoptosis and NF-κB activation in many cell types.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.