Thermo Fisher Scientific TRAIL (soluble) Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sTRAIL/Apo2L. Anti-Human sTRAIL/Apo2L-specific antibody was purified by affinity chromatography employing an immobilized Human sTRAIL/Apo2L matrix.

Sandwich ELISA:
To detect Human sTRAIL/Apo2L by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sTRAIL/Apo2L (500-P135Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human sTRAIL/Apo2L.

Western Blot:
To detect Human sTRAIL/Apo2L by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Human sTRAIL/Apo2L is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

TRAIL is a cytokine belonging to the tumor necrosis factor (TNF) ligand family. It preferentially induces apoptosis in transformed and tumor cells but does not appear to kill normal cells, although expressed at significant levels in most normal tissues.
TRAIL binds to several TNF receptor superfamily members including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly TNFRSF11B/OPG.
Its activity may be modulated by binding to decoy receptors (TRAILR3, TRAILR4, OPG) that cannot induce apoptosis.
Binding of TRAIL to its receptors triggers activation of MAPK8/JNK, caspase 8, and caspase 3.
Receptors include death domain-containing DR4 and DR5, and decoy receptors DcR1 and DcR2.
TRAIL is a type II membrane protein expressed in various human tissues and induces apoptosis and NF-κB activation similar to TNF and Fas ligand.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.