Thermo Fisher Scientific SETD8 Monoclonal Antibody (OTI3G9), TrueMAB
SETD8 단백질을 인식하는 마우스 단클론 항체로, Western blot 및 IHC(P) 실험에 최적화됨. 인간 단백질에 반응하며, 동결건조 형태로 제공. PBS 버퍼에 8% 트레할로스 포함, 보존제 없음. -20°C에서 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3G9 |
| Immunogen | Full length human recombinant protein of human SETD8 produced in E.coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
SETD8 is a histone methyltransferase that specifically monomethylates Lys-20 of histone H4. This monomethylation is enriched during mitosis and serves as a marker for epigenetic transcriptional repression. SETD8 functions mainly in euchromatin regions, playing a central role in silencing euchromatic genes and maintaining proper DNA structure during mitosis. It is also involved in chromosome condensation and cytokinesis. Nucleosomes are preferred substrates compared to free histones.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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