Thermo Fisher Scientific Phospho-AKT1/AKT2/AKT3 (Thr308) Polyclonal Antibody
AKT1/2/3 단백질의 Thr308 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF에 적합하며 인간, 생쥐, 랫트 반응성. 고순도 액상 형태로 제공되며 -20°C 보관.
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Thermo Fisher Scientific Phospho-AKT1/AKT2/AKT3 (Thr308) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human AKT1 (Accession P31749), corresponding to amino acid residues around phosphorylated Thr308 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816342 |
Product Specific Information
This antibody detects endogenous levels of pan-AKT1/2/3 only when phosphorylated at Threonine 308.
Target Information
AKT, also known as protein kinase B (PKB) or RAS-alpha, is a serine/threonine kinase involved in regulation of metabolism, cell survival, and growth. It is activated by insulin, PI3K, IGF1, and other growth factors, promoting cell survival by inhibiting apoptosis through phosphorylation and inactivation of targets such as forkhead transcription factors and caspase-9. The AKT pathway is a major focus in cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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