Thermo Fisher Scientific CYPA Polyclonal Antibody
Thermo Fisher Scientific CYPA Polyclonal Antibody는 인간, 마우스, 랫트 시료에 반응하는 Rabbit IgG 폴리클로날 항체입니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합하며, Protein A로 정제된 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific CYPA Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human Cyclophilin A, amino acids 101–165 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents — cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs), and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes inhibit calcineurin, a calcium and calmodulin-dependent protein phosphatase involved in T-cell activation, providing a mechanism of immunosuppression by CsA and FK506.
Immunophilins function as peptidyl-prolyl cis-trans isomerases (PPIase), accelerating protein folding by catalyzing slow steps in proline-containing proteins. Within the cyclophilin family, CyPA, CyPB, and CyPC show high homology. CyPA (also termed CyP-18) is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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