Thermo Fisher Scientific Cytokeratin 14 Recombinant Rabbit Monoclonal Antibody (ARC0351)
Cytokeratin 14을 인식하는 재조합 토끼 단일클론 항체로, Western blot, IHC(P), ELISA에 사용 가능. 인간, 마우스, 랫트 반응성. HEK293 세포 발현, IgG 형식. -20°C 보관, 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:800 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0351 |
| Immunogen | Recombinant fusion protein containing amino acids 264–457 of human Cytokeratin 14 (KRT14, P02533) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849124 |
Product Specific Information
Immunogen sequence:
VGGDVNVEMD AAPGVDLSRI LNEMRDQYEK MAEKNRKDAE EWFFTKTEEL NREVATNSEL VQSGKSEISE LRRTMQNLEI ELQSQLSMKA SLENSLEETK GRYCMQLAQI QEMIGSVEEQ LAQLRCEMEQ QNQEYKILLD VKTRLEQEIA TYRRLLEGED AHLSSSQFSS GSQSSRDVTS SSRQIRTKVM DVHD
Target Information
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissues (e.g., skin) and non-keratinizing cells (e.g., mesothelial cells). At least 20 different CKs can be identified.
Most CK family members fall into two classes: type I (acidic polypeptides) and type II (basic polypeptides).
Keratin 14, a type I low molecular weight keratin, exists in combination with keratin 5 and distinguishes stratified epithelial cells from simple epithelial cells. It is useful in identifying squamous cell carcinomas and is considered a prognostic marker in breast carcinomas.
At least one member of the acidic family and one of the basic family is expressed in all epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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