Thermo Fisher Scientific Cyclin B1 Recombinant Rabbit Monoclonal Antibody (ARC0474)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,500-1:10,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC0474

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 3-177 of human Cyclin B1 (P14635) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cyclin B1,uniProtId:P14635-1,ncbiNodeId:9606,antigenRange:3-177,antigenLength:433,antigenImageFileName:MA5-35219_Cyclin_B1_P14635-1_Rabbit.svg,antigenImageFileNamePDP:MA5-35219_Cyclin_B1_P14635-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849123

Product Specific Information

Immunogen sequence: LRVTRNSKIN AENKAKINMA GAKRVPTAPA ATSKPGLRPR TALGDIGNKV SEQLQAKMPM KKEAKPSATG KVIDKKLPKP LEKVPMLVPV PVSEPVPEPE PEPEPEPVKE EKLSPEPILV DTASPSPMET SGCAPAEEDL CQAFSDVILA VNDVDAEDGA DPNLCSEYVK DIYAY

Target Information

Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.