Thermo Fisher Scientific Cyclin B1 Recombinant Rabbit Monoclonal Antibody (ARC0474)
Cyclin B1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot 및 ELISA에 적합합니다. 인간과 마우스 시료에 반응하며, 세포 주기 G2/M 전이 조절 연구에 활용됩니다. 고순도 친화 크로마토그래피 정제, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,500–1:10,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0474 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 3–177 of human Cyclin B1 (P14635) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849123 |
Product Specific Information
Immunogen sequence:
LRVTRNSKIN AENKAKINMA GAKRVPTAPA ATSKPGLRPR TALGDIGNKV SEQLQAKMPM KKEAKPSATG KVIDKKLPKP LEKVPMLVPV PVSEPVPEPE PEPEPEPVKE EKLSPEPILV DTASPSPMET SGCAPAEEDL CQAFSDVILA VNDVDAEDGA DPNLCSEYVK DIYAY
Target Information
Cyclins bind to and regulate the activity of Cyclin-dependent protein kinases (CDKs). Cyclin B1 (CCNB1) is a regulatory protein involved in mitosis and is essential for controlling the cell cycle at the G2/M transition. It forms the maturation-promoting factor (MPF) complex with p34 (cdc2). Two alternative transcripts exist: one constitutively expressed and another cell cycle-regulated, predominantly during the G2/M phase. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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